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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 353307 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#353307, RRID:AB_11149688
- Product name
- Brilliant Violet 421™ anti-human Perforin [B-D48]
- Antibody type
- Monoclonal
- Antigen
- Perforin
- Description
- Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Blue dye
- Isotype
- IgG
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral lymphocytes were surface stained with CD56 PE and then intracellularly stained with perforin (clone B-D48) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
- Conjugate
- Blue dye
