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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-12469 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Perforin Monoclonal Antibody (5B10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-12469 targets Perforin in IHC (P) applications and shows reactivity with Human samples.
- Antibody clone number
- 5B10
- Concentration
- Conc. Not Determined
Submitted references Tissue Damage in Human Cutaneous Leishmaniasis: Correlations Between Inflammatory Cells and Molecule Expression.
Recurrent lipoatrophic panniculitis of children.
Functions of liver natural killer cells are dependent on the severity of liver inflammation and fibrosis in chronic hepatitis C.
Immunohistochemical expression of granzyme B and perforin in discoid lupus erythematosus.
Primary angiitis of the central nervous system: 2 atypical cases.
Adrenal extranodal NK/T-cell lymphoma diagnosed by fine-needle aspiration and cerebrospinal fluid cytology and immunophenotyping: a case report.
Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy--a case report.
GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy.
Saldanha MG, Pagliari C, Queiroz A, Machado PRL, Carvalho L, Scott P, Carvalho EM, Arruda S
Frontiers in cellular and infection microbiology 2020;10:355
Frontiers in cellular and infection microbiology 2020;10:355
Recurrent lipoatrophic panniculitis of children.
Torrelo A, Noguera-Morel L, Hernández-Martín A, Clemente D, Barja JM, Buzón L, Azorín D, de Jesús AA, López-Robledillo JC, Colmenero I, Kutzner H, Goldbach-Mansky R, Requena L
Journal of the European Academy of Dermatology and Venereology : JEADV 2017 Mar;31(3):536-543
Journal of the European Academy of Dermatology and Venereology : JEADV 2017 Mar;31(3):536-543
Functions of liver natural killer cells are dependent on the severity of liver inflammation and fibrosis in chronic hepatitis C.
Fugier E, Marche H, Thélu MA, Macek Jílková Z, Van Campenhout N, Dufeu-Duchesne T, Leroy V, Zarski JP, Sturm N, Marche PN, Jouvin-Marche E
PloS one 2014;9(4):e95614
PloS one 2014;9(4):e95614
Immunohistochemical expression of granzyme B and perforin in discoid lupus erythematosus.
Abdou AG, Shoeib M, Bakry OA, El-Bality H
Ultrastructural pathology 2013 Dec;37(6):408-16
Ultrastructural pathology 2013 Dec;37(6):408-16
Primary angiitis of the central nervous system: 2 atypical cases.
Pagni F, Isimbaldi G, Vergani F, Casiraghi P, Marzorati L, Migliorino G, Cattoretti G
Folia neuropathologica 2012;50(3):293-9
Folia neuropathologica 2012;50(3):293-9
Adrenal extranodal NK/T-cell lymphoma diagnosed by fine-needle aspiration and cerebrospinal fluid cytology and immunophenotyping: a case report.
Dunning KK, Wudhikarn K, Safo AO, Holman CJ, McKenna RW, Pambuccian SE
Diagnostic cytopathology 2009 Sep;37(9):686-95
Diagnostic cytopathology 2009 Sep;37(9):686-95
Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy--a case report.
Milani V, Stangl S, Issels R, Gehrmann M, Wagner B, Hube K, Mayr D, Hiddemann W, Molls M, Multhoff G
Journal of translational medicine 2009 Jun 23;7:50
Journal of translational medicine 2009 Jun 23;7:50
GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy.
van Es LA, de Heer E, Vleming LJ, van der Wal A, Mallat M, Bajema I, Bruijn JA, de Fijter JW
Kidney international 2008 Jun;73(12):1426-33
Kidney international 2008 Jun;73(12):1426-33
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of NK-92 (Lane 1) and NK-92 treated with IL-2 (100 ng/mL for 48hrs) (Lane 2). The blot was probed with Anti-Perforin Mouse Monoclonal Antibody (Product # MA5-12469, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 61 kDa band corresponding to Perforin was detected in NK-92 cells upon IL-2 treatment.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of NK-92 (Lane 1) and NK-92 treated with IL-2 (100 ng/mL for 48hrs) (Lane 2). The blot was probed with Anti-Perforin Mouse Monoclonal Antibody (Product # MA5-12469, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 61 kDa band corresponding to Perforin was detected in NK-92 cells upon IL-2 treatment.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human lymphoma stained with Perforin antibody using peroxidase-conjugate and AEC chromogen. Note granular cytoplasmic staining of lymphocytes.
