Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ALX-804-135-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-135-C100, RRID:AB_2052203
- Product name
- Perforin monoclonal antibody (CE2.10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CE2.10
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Detection of perforin in the mouse T cell clone B6.1. (lane 2). As control cell line HEK 293 was used (lane 1). The protein migrates around ~66kDa. Method: Cell extracts from the T cell clone B6.1 (2x10E6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10 antibody at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.