16-9927-050

antibody from Invitrogen Antibodies

Targeting: TNFSF10 Apo-2L, CD253, TL2, TRAIL

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16-9927-050

Provider

Invitrogen Antibodies

Product name

CD253 (TRAIL) Monoclonal Antibody (RIK-2), Functional Grade, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The RIK-2 monoclonal antibody reacts with human TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not expressed by resting human cells but has been shown to be induced under certain activation conditions. Iin addition it is expressed on several human tumor lines. Interaction of TRAIL with its ligand, Apo-2, induces apoptosis. RIK-2 blocks TRAIL-induced apoptosis.

Antibody clone number

RIK-2

Concentration

1 mg/mL

References

Submitted references

Canonical TGF-β Signaling Pathway Represses Human NK Cell Metabolism.

Zaiatz-Bittencourt V, Finlay DK, Gardiner CM
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 15;200(12):3934-3941

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MADD knock-down enhances doxorubicin and TRAIL induced apoptosis in breast cancer cells.

Turner A, Li LC, Pilli T, Qian L, Wiley EL, Setty S, Christov K, Ganesh L, Maker AV, Li P, Kanteti P, Das Gupta TK, Prabhakar BS
PloS one 2013;8(2):e56817

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Dengue virus activates membrane TRAIL relocalization and IFN-α production by human plasmacytoid dendritic cells in vitro and in vivo.

Gandini M, Gras C, Azeredo EL, Pinto LM, Smith N, Despres P, da Cunha RV, de Souza LJ, Kubelka CF, Herbeuval JP
PLoS neglected tropical diseases 2013;7(6):e2257

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TRAIL death receptor-4, decoy receptor-1 and decoy receptor-2 expression on CD8+ T cells correlate with the disease severity in patients with rheumatoid arthritis.

Bisgin A, Terzioglu E, Aydin C, Yoldas B, Yazisiz V, Balci N, Bagci H, Gorczynski RM, Akdis CA, Sanlioglu S
BMC musculoskeletal disorders 2010 Aug 27;11:192

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Bystander macrophage apoptosis after Mycobacterium tuberculosis H37Ra infection.

Kelly DM, ten Bokum AM, O'Leary SM, O'Sullivan MP, Keane J
Infection and immunity 2008 Jan;76(1):351-60

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HIV turns plasmacytoid dendritic cells (pDC) into TRAIL-expressing killer pDC and down-regulates HIV coreceptors by Toll-like receptor 7-induced IFN-alpha.

Hardy AW, Graham DR, Shearer GM, Herbeuval JP
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 30;104(44):17453-8

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Anti-HIV state but not apoptosis depends on IFN signature in CD4+ T cells.

Audigé A, Urosevic M, Schlaepfer E, Walker R, Powell D, Hallenberger S, Joller H, Simon HU, Dummer R, Speck RF
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 1;177(9):6227-37

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of human Trail transfected cells with Anti-Human CD253 (TRAIL) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 TRAIL and its receptor (both death and decoy) expression profile on CD4 + T cells . A scattered dot plot profile of TRAIL and its receptors is provided in Panel A . Solid circles indicate RA patients while dots represent control individuals. Error bars display mean (+-) SEM. Representative flow cytometric analyses of a control individual (upper panels) and an RA patient (lower panels) are shown in Panel B , while Panel C shows the relative increase in TRAIL and its receptors on CD4 + T cells of RA patients compared to controls.