ALX-804-300-C100

antibody from Enzo Life Sciences

Targeting: TNFSF10 Apo-2L, CD253, TL2, TRAIL

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ALX-804-300-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-300-C100, RRID:AB_2052720

Product name

TRAIL (human) monoclonal antibody (HS501)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HS501

Vial size

100 μg

Storage

-80°C

Handling

Thaw at +37¡C. Avoid freeze/thaw cycles.

References

Submitted references

TNF-related apoptosis-inducing ligand mediates tumoricidal activity of human monocytes stimulated by Newcastle disease virus.

Washburn B, Weigand MA, Grosse-Wilde A, Janke M, Stahl H, Rieser E, Sprick MR, Schirrmacher V, Walczak H
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 15;170(4):1814-21

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Equal numbers of Jurkat cells treated and untreated with IFNa compared with equal numbers of CV1-EBNA cells transfected with human TRAIL and a control expression plasmid (not encoding hTRAIL) were analyzed by using HS501 (Prod. No. ALX-804-300. Method: Jurkat cells were treated with IFNa [1000 IU/ml] for 8 hours (lane 1) or were left untreated (lane 2), and CV1-EBNA cells were transfected with pcDNA3 expression vector encoding human TRAIL (lane 3), or with human TRAIL-R1 (lane 4). Lysates of equal numbers of cells were analyzed by Western blot with anti-TRAIL HS501 (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by horseradish peroxidase-conjugated anti-mouse IgG1 antibody and developed by ECL.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Equal numbers of CV1-EBNA cells transfected with control expression plasmids (negative for human TRAIL) were analyzed and compared to a positive control cell-lysate using HS501. Method: CV1-EBNA cells, either transfected with pcDNA3 expression vector encoding TRAIL-R1 to TRAIL-R4 (lanes 1-4), or with human TRAIL (lane 5) were analyzed with HS501 MAb against TRAIL (Prod. No. ALX-804-300). The primary antibody was used at 1µg/ml, followed by a horseradish peroxidase conjugated anti-mouse IgG1 antibody and developed by ECL.