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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX60830 - Provider product page
- Provider
- GeneTex
- Product name
- HDAC1 antibody [GT9078]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of nuclear extracts from HeLa cells (40 £gg) using HDAC1 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ChIP was performed with HeLa extracts and either control IgG (1 £gg) or HDAC1 antibody (3 and 12 £gl per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter and the coding region of p21.
