Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX60831 - Provider product page
- Provider
- GeneTex
- Product name
- HDAC1 antibody [GT9079]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of nuclear extracts from HeLa cells (40 £gg) using HDAC1 antibody at a dilution of 1:2,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with HeLa extracts and either control IgG (5 £gg) or HDAC1 antibody (1, 2, 5, and 10 £gg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter and the coding region of p21.
