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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- TA319251 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-HDAC-1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-HDAC-1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HDAC1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.33 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Lane 2: none. Load: 50 ug per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55kDa for HDAC-1. Other band(s): none.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4°C. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none. .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF of Rabbit Anti-HDAC-1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 was used with Atto 425 (shown in red). Anti-Keratin monoclonal antibody was used with Dylight 488 (shown in green) to detect Keratin. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence Microscopy of Rabbit anti-HDAC1 Antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum. Primary antibody: HDAC1 antibody at 04 ug/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT. Localization: HDAC1 is nuclear. Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 as brown color indicates presence of protein, blue color shows cell nuclei.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain.
- Validation comment
- IHC
