TA324515

antibody from OriGene

Targeting: HDAC1 GON-10, HD1, KDAC1, RPD3L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324515

Provider

OriGene

Product name

Rabbit polyclonal anti-HDAC1 antibody, Loading control

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-HDAC1 antibody, Loading control

Host

Rabbit

Conjugate

Unconjugated

Epitope

HDAC1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HDAC1 Antibody (N-term) (Cat. #TA324515) western blot analysis in MCF-7 cell line and human placenta and normal uterus,rat spleen lysates (35ug/lane).This demonstrates the HDAC1 antibody detected the HDAC1 protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of MCF-7 cell stained with HDAC1 Antibody (N-term)(Cat#TA324515).MCF-7 cells were incubated with HDAC1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . HDAC1 immunoreactivity is localized to Nucleus significantly.

Validation comment

IF