Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324609 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal HDAC1 Antibody (Center S423)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal HDAC1 Antibody (Center S423)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HDAC1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.45 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HDAC1 Antibody (S423) (Cat. #TA324609) western blot analysis in Hela cell line and mouse testis,rat testis tissue lysates (35ug/lane).This demonstrates the HDAC1 antibody detected the HDAC1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with HDAC1 Antibody (Center S423)(Cat#TA324609).Hela cells were incubated with HDAC1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . HDAC1 immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
