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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Chromatin Immunoprecipitation [1]
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- Product number
- R34560 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HDAC1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids KPEAKGVKEEVK were used as the immunogen for this HDAC1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the HDAC1 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa lysate with HDAC1 antibody at 0.5ug/ml. Predicted molecular weight 55~60 kDa. Larger size likely due to sumoylation.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) duodenum and 2) lymph node lysate with HDAC1 antibody at 0.1ug/ml. Predicted molecular weight 55~60 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse NIH3T3 lysate with HDAC1 antibody at 0.1ug/ml. Predicted molecular weight 55~60 kDa. Larger size likely due to sumoylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ChIP of 2ug of HDAC1 antibody with 1ug MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.
