PA1-860-A647

antibody from Invitrogen Antibodies

Targeting: HDAC1 GON-10, HD1, KDAC1, RPD3L1

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-860-A647

Provider

Invitrogen Antibodies

Product name

HDAC1 Polyclonal Antibody, Alexa Fluor™ 647

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1860A647 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.

Conjugate

Red dye

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HDAC1 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HDAC1 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-860-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Red dye