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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA502142 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502142, RRID:AB_11126810
- Product name
- HDAC1 mouse monoclonal antibody, clone OTI5C3 (formerly 5C3)
- Antibody type
- Monoclonal
- Description
- HDAC1 mouse monoclonal antibody, clone OTI5C3 (formerly 5C3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HDAC1
- Isotype
- IgG
- Antibody clone number
- OTI5C3
- Vial size
- 100 µl
- Concentration
- 0.43 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC1 (RC201745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC1 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HDAC1 mouse monoclonal antibody (TA502142) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC1(RC201745).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201745 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HDAC1 antibody(TA502142), and then analyzed by flow cytometry.
- Validation comment
- FC
