PA5-120036

antibody from Invitrogen Antibodies

Targeting: HDAC1 GON-10, HD1, KDAC1, RPD3L1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120036

Provider

Invitrogen Antibodies

Product name

HDAC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: 293T, HeLa, HepG2, MCF7, Mouse thymus, Mouse testis, Rat thymus, Rat testis . The target is usually found in the following locations: Nucleus. Immunogen sequence: SGDEDEDDPD KRISICSSDK RIACEEEFSD SEEEGEGGRK NSSNFKKAKR VKTEDEKEKD PEEKKEVTEE EKTKEEKPEA KGVKEEVKLA

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.55 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HDAC1 in extracts of various cell lines. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HDAC1 in extracts from normal (control) and HDAC1 knockout (KO) 293T cells. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HDAC1 in A549 cells. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of HDAC1 in A549 cells using a HDAC1 Polyclonal antibody (Product # PA5-120036). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence (Immunocytochemistry) analysis of HDAC1 in L929 cells. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:200. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HDAC1 in 200 μg extracts of HT-29 cells. Samples were precipitated with 3 μg HDAC1 Polyclonal antibody (Product # PA5-120036). Western blot was performed from the immunoprecipitate using HDAC1 Polyclonal antibody (Product # PA5-120036) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human colon. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human lung cancer. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDAC1 in paraffin-embedded rat spleen. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human lung cancer. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDAC1 in paraffin-embedded rat spleen. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HDAC1 in paraffin-embedded human colon. Samples were incubated with HDAC1 Polyclonal antibody (Product # PA5-120036) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.