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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NBP1-78101 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78101, RRID:AB_11036899
- Product name
- Rabbit Polyclonal HDAC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.33 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Epigenetic effects of chromatin remodeling agents on organotypic cultures.
Sirchia SM, Faversani A, Rovina D, Russo MV, Paganini L, Savi F, Augello C, Rosso L, Del Gobbo A, Tabano S, Bosari S, Miozzo M
Epigenomics 2016 Mar;8(3):341-58
Epigenomics 2016 Mar;8(3):341-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC1 Antibody [NBP1-78101] - Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. .
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC1 Antibody [NBP1-78101] - Analysis of LNCaP prostate cancer cells. Lysate was loaded at 50ug per lane and incubated with 1:1000 dilution of primary Ab. A band was detected at the expected molecular weight of 55 Kda.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-78101] - HDAC1 antibody shows strong nuclear staining of tumor cells in human lung tissue. Brown color indicates presence of protein, blue color shows cell nuclei.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Analysis of Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain.
