Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-83350 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83350, RRID:AB_11041279
- Product name
- Rabbit Polyclonal HDAC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human HDAC1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Recruitment of Oct4 protein to UV-damaged chromatin in embryonic stem cells.
Bártová E, Šustáčková G, Stixová L, Kozubek S, Legartová S, Foltánková V
PloS one 2011;6(12):e27281
PloS one 2011;6(12):e27281
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC1 Antibody [NBP1-83350] - Analysis in human cell line HeLa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC1 Antibody [NBP1-83350] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human duodenum shows nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human colon shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining in human colon and skeletal muscle tissues using anti-HDAC1 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.