Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-56340 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56340, RRID:AB_838328
- Product name
- Rabbit Polyclonal HDAC1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. In 293, a 60 kDa band is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The long noncoding RNA ROCKI regulates inflammatory gene expression.
Chemical and genetic control of IFNγ-induced MHCII expression.
HDAC1 and HDAC2 collectively regulate intestinal stem cell homeostasis.
G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition.
Sin3a-associated Hdac1 and Hdac2 are essential for hematopoietic stem cell homeostasis and contribute differentially to hematopoiesis.
Dosage-dependent tumor suppression by histone deacetylases 1 and 2 through regulation of c-Myc collaborating genes and p53 function.
Too much or too little, how much HDAC activity is good for you?
Tolerogenic dendritic cells induce CD4+CD25hiFoxp3+ regulatory T cell differentiation from CD4+CD25-/loFoxp3- effector T cells.
Overlapping functions of Hdac1 and Hdac2 in cell cycle regulation and haematopoiesis.
HDAC4 promotes growth of colon cancer cells via repression of p21.
Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer.
Zhang Q, Chao TC, Patil VS, Qin Y, Tiwari SK, Chiou J, Dobin A, Tsai CM, Li Z, Dang J, Gupta S, Urdahl K, Nizet V, Gingeras TR, Gaulton KJ, Rana TM
The EMBO journal 2019 Apr 15;38(8)
The EMBO journal 2019 Apr 15;38(8)
Chemical and genetic control of IFNγ-induced MHCII expression.
Wijdeven RH, van Luijn MM, Wierenga-Wolf AF, Akkermans JJ, van den Elsen PJ, Hintzen RQ, Neefjes J
EMBO reports 2018 Sep;19(9)
EMBO reports 2018 Sep;19(9)
HDAC1 and HDAC2 collectively regulate intestinal stem cell homeostasis.
Zimberlin CD, Lancini C, Sno R, Rosekrans SL, McLean CM, Vlaming H, van den Brink GR, Bots M, Medema JP, Dannenberg JH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 May;29(5):2070-80
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 May;29(5):2070-80
G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition.
Laumet G, Garriga J, Chen SR, Zhang Y, Li DP, Smith TM, Dong Y, Jelinek J, Cesaroni M, Issa JP, Pan HL
Nature neuroscience 2015 Dec;18(12):1746-55
Nature neuroscience 2015 Dec;18(12):1746-55
Sin3a-associated Hdac1 and Hdac2 are essential for hematopoietic stem cell homeostasis and contribute differentially to hematopoiesis.
Heideman MR, Lancini C, Proost N, Yanover E, Jacobs H, Dannenberg JH
Haematologica 2014 Aug;99(8):1292-303
Haematologica 2014 Aug;99(8):1292-303
Dosage-dependent tumor suppression by histone deacetylases 1 and 2 through regulation of c-Myc collaborating genes and p53 function.
Heideman MR, Wilting RH, Yanover E, Velds A, de Jong J, Kerkhoven RM, Jacobs H, Wessels LF, Dannenberg JH
Blood 2013 Mar 14;121(11):2038-50
Blood 2013 Mar 14;121(11):2038-50
Too much or too little, how much HDAC activity is good for you?
Matthias P
Blood 2013 Mar 14;121(11):1930-1
Blood 2013 Mar 14;121(11):1930-1
Tolerogenic dendritic cells induce CD4+CD25hiFoxp3+ regulatory T cell differentiation from CD4+CD25-/loFoxp3- effector T cells.
Huang H, Dawicki W, Zhang X, Town J, Gordon JR
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 1;185(9):5003-10
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 1;185(9):5003-10
Overlapping functions of Hdac1 and Hdac2 in cell cycle regulation and haematopoiesis.
Wilting RH, Yanover E, Heideman MR, Jacobs H, Horner J, van der Torre J, DePinho RA, Dannenberg JH
The EMBO journal 2010 Aug 4;29(15):2586-97
The EMBO journal 2010 Aug 4;29(15):2586-97
HDAC4 promotes growth of colon cancer cells via repression of p21.
Wilson AJ, Byun DS, Nasser S, Murray LB, Ayyanar K, Arango D, Figueroa M, Melnick A, Kao GD, Augenlicht LH, Mariadason JM
Molecular biology of the cell 2008 Oct;19(10):4062-75
Molecular biology of the cell 2008 Oct;19(10):4062-75
Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer.
Wilson AJ, Byun DS, Popova N, Murray LB, L'Italien K, Sowa Y, Arango D, Velcich A, Augenlicht LH, Mariadason JM
The Journal of biological chemistry 2006 May 12;281(19):13548-58
The Journal of biological chemistry 2006 May 12;281(19):13548-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.