AP09288PU-N

antibody from Acris Antibodies GmbH

Targeting: HDAC1 GON-10, HD1, KDAC1, RPD3L1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09288PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09288PU-N, RRID:AB_2035620

Product name

anti HDAC1 (466-482)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 466-482 of Human HDAC-1

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.33 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-HDAC-1 antibody shows detection of a band at ~65 kDa corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 µg of lysate wseparated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeas (TM)800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Affinity Purified anti-HDAC-1 antibody was used at a 1:500 dilution to detect HDAC-1 by immunohistochemistry in human prostate cancer tissue. Tissue was formalin-fixed and paraffin embedded.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Affinity Purified anti-HDAC1 antibody shows strong nuclear staining of tumor cells in human lung tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.