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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- ABIN1107492 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Histone Deacetylase 1 (HDAC1) (Internal Region), (AA 385-396) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region of the protein sequence according to NP_032254.1. Genename: Hdac1
- Description
- Affinity Chromatography
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Goat
- Antigen sequence
C-PEDAIPEESGDE- Epitope
- Internal Region,AA 385-396
- Vial size
- 0.1 mg
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP31933PU-N HDAC1 antibody staining of NIH3T3 lysate at 0.3 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ChIP of 2 μg AP31933PU-N with MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.
