600-401-845

antibody from Rockland Immunochemicals, Inc.

Targeting: GLI2 HPE9, THP1, THP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-845

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-845, RRID:AB_2111910

Product name

Anti-GLI2 (RABBIT) Antibody - 600-401-845

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Patched 1 Expression Correlates with Biochemical Relapse in High-Risk Prostate Cancer Patients.

Gonnissen A, Isebaert S, Perneel C, McKee CM, Van Utterbeeck F, Lerut E, Verrill C, Bryant RJ, Joniau S, Muschel RJ, Haustermans K
The American journal of pathology 2018 Mar;188(3):795-804

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Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer.

Gonnissen A, Isebaert S, Perneel C, McKee CM, Verrill C, Bryant RJ, Van Utterbeeck F, Lerut E, Haustermans K, Muschel RJ
BMC cancer 2017 Sep 6;17(1):634

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The Effect of Metformin and GANT61 Combinations on the Radiosensitivity of Prostate Cancer Cells.

Gonnissen A, Isebaert S, McKee CM, Muschel RJ, Haustermans K
International journal of molecular sciences 2017 Feb 13;18(2)

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The hedgehog inhibitor GANT61 sensitizes prostate cancer cells to ionizing radiation both in vitro and in vivo.

Gonnissen A, Isebaert S, McKee CM, Dok R, Haustermans K, Muschel RJ
Oncotarget 2016 Dec 20;7(51):84286-84298

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead).  See Ruppert et al for testing conditions.  Each lane contains approximately 35 µg of lysate.  Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4°C.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (611-132-122) for 45 min at room temperature (800 nm channel, green).   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).   IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot