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- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-17070 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- E2F1 Monoclonal Antibody (8G9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17070 targets E2F1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples. The MA5-17070 immunogen is purified recombinant fragment of human E2F1 (amino acids: 69-223) expressed in E. Coli. MA5-17070 detects E2F1 which has a predicted molecular weight of approximately 46.9kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8G9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of E2F1 using a E2F1 monoclonal antibody (Product # MA5-17070) against a human E2F1 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of E2F1 using E2F1 monoclonal antibody (Product # MA5-17070) in HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of E2F1 using E2F1 monoclonal antibody (Product # MA5-17070) in HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of E2F1 using a E2F1 monoclonal antibody (Product # MA5-17070) against a human E2F1 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of E2F1 using E2F1 monoclonal antibody (Product # MA5-17070) in HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 monoclonal antibody (Product # MA5-17070) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 monoclonal antibody (Product # MA5-17070) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using E2F1 monoclonal antibody (Product # MA5-17070) (green) and negative control (red).
