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- Antibody Data
- Antigen structure
- References [45]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 12171-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12171-1-AP, RRID:AB_2096958
- Product name
- E2F1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated E2F1 antibody (Cat. #12171-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester).
CDKN2A Deletion in Melanoma Excludes T Cell Infiltration by Repressing Chemokine Expression in a Cell Cycle-Dependent Manner.
SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1.
Nucleolar and spindle‑associated protein 1 promotes non‑small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D.
Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes.
Knockdown of long non‑coding RNA ANRIL inhibits the proliferation and promotes the apoptosis of Burkitt lymphoma cells through the TGF‑β1 signaling pathway.
The application of CRISPR/Cas9 system in cervical carcinogenesis.
MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.
MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI.
CDK4/6 inhibitor palbociclib overcomes acquired resistance to third-generation EGFR inhibitor osimertinib in non-small cell lung cancer (NSCLC).
CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.
Apolipoprotein E2 modulates cell cycle function to promote proliferation in pancreatic cancer cells via regulation of the c-Myc-p21Waf1 signalling pathway.
Long noncoding RNA FBXL19-AS1 induces tumor growth and metastasis by sponging miR-203a-3p in lung adenocarcinoma.
S-Adenosylmethionine Affects Cell Cycle Pathways and Suppresses Proliferation in Liver Cells.
Bromodomain inhibitor jq1 induces cell cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway.
Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.
Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway.
Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.
Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.
Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.
Roles of B7-H3 in Cervical Cancer and Its Prognostic Value.
Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy.
Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.
Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.
TACC3 transcriptionally upregulates E2F1 to promote cell growth and confer sensitivity to cisplatin in bladder cancer.
Whole exome-wide association study identifies a missense variant in SLC2A4RG associated with glioblastoma risk.
Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.
The transcription factor FOXA1 induces epithelial ovarian cancer tumorigenesis and progression.
AhR‑E2F1‑KGFR signaling is involved in KGF‑induced intestinal epithelial cell proliferation.
RACK1 promotes lung cancer cell growth via an MCM7/RACK1/ Akt signaling complex.
In vitro and ex vivo delivery of tailored siRNA-nanoliposomes for E2F1 silencing as a potential therapy for colorectal cancer.
Roles of immune inhibitory molecule B7-H4 in cervical cancer.
Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.
Dihydroartemisinin suppresses pancreatic cancer cells via a microRNA-mRNA regulatory network.
SUMOylation of Rb enhances its binding with CDK2 and phosphorylation at early G1 phase.
Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1).
CIP2A is associated with multidrug resistance in cervical adenocarcinoma by a P-glycoprotein pathway.
miR-148a-3p represses proliferation and EMT by establishing regulatory circuits between ERBB3/AKT2/c-myc and DNMT1 in bladder cancer.
Regulation of Hepatic Cholesteryl Ester Transfer Protein Expression and Reverse Cholesterol Transport by Inhibition of DNA Topoisomerase II.
TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.
The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.
Notch1 is a potential therapeutic target for the treatment of human hepatitis B virus X protein-associated hepatocellular carcinoma.
Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.
Hepatitis B virus X protein inhibits tumor suppressor miR-205 through inducing hypermethylation of miR-205 promoter to enhance carcinogenesis.
The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.
Xiong J, Tan S, Yu L, Shen H, Qu S, Zhang C, Ren C, Zhu D, Wang H
International journal of nanomedicine 2021;16:7609-7622
International journal of nanomedicine 2021;16:7609-7622
CDKN2A Deletion in Melanoma Excludes T Cell Infiltration by Repressing Chemokine Expression in a Cell Cycle-Dependent Manner.
Zhu Z, Song H, Xu J
Frontiers in oncology 2021;11:641077
Frontiers in oncology 2021;11:641077
SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1.
Ma J, He Z, Zhang H, Zhang W, Gao S, Ni X
Cell death & disease 2021 May 27;12(6):550
Cell death & disease 2021 May 27;12(6):550
Nucleolar and spindle‑associated protein 1 promotes non‑small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D.
Ling B, Wei P, Xiao J, Cen B, Wei H, Feng X, Ye G, Li S, Zhang Z, Liang W, Huang S, Huang W
Oncology reports 2021 Mar;45(3):1044-1058
Oncology reports 2021 Mar;45(3):1044-1058
Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes.
Sun X, Fu P, Xie L, Chai S, Xu Q, Zeng L, Wang X, Jiang N, Sang M
International journal of molecular medicine 2021 Jan;47(1):335-345
International journal of molecular medicine 2021 Jan;47(1):335-345
Knockdown of long non‑coding RNA ANRIL inhibits the proliferation and promotes the apoptosis of Burkitt lymphoma cells through the TGF‑β1 signaling pathway.
Mao S, Jin J, Li Z, Yang W
Molecular medicine reports 2021 Feb;23(2)
Molecular medicine reports 2021 Feb;23(2)
The application of CRISPR/Cas9 system in cervical carcinogenesis.
Gao C, Wu P, Yu L, Liu L, Liu H, Tan X, Wang L, Huang X, Wang H
Cancer gene therapy 2021 Aug 4;
Cancer gene therapy 2021 Aug 4;
MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.
Chen F, Wang Q, Yu X, Yang N, Wang Y, Zeng Y, Zheng Z, Zhou F, Zhou Y
Cell death & disease 2021 Apr 6;12(4):370
Cell death & disease 2021 Apr 6;12(4):370
MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI.
Wei X, Wang B, Wang Q, Yang X, Yang Y, Fang Z, Yi C, Shi L, Fan X, Tao J, Guo Y, Song D
Frontiers in pharmacology 2020;11:164
Frontiers in pharmacology 2020;11:164
CDK4/6 inhibitor palbociclib overcomes acquired resistance to third-generation EGFR inhibitor osimertinib in non-small cell lung cancer (NSCLC).
Qin Q, Li X, Liang X, Zeng L, Wang J, Sun L, Zhong D
Thoracic cancer 2020 Sep;11(9):2389-2397
Thoracic cancer 2020 Sep;11(9):2389-2397
CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.
Ying Y, Li J, Xie H, Yan H, Jin K, He L, Ma X, Wu J, Xu X, Fang J, Wang X, Zheng X, Liu B, Xie L
Cell proliferation 2020 Feb;53(2):e12751
Cell proliferation 2020 Feb;53(2):e12751
Apolipoprotein E2 modulates cell cycle function to promote proliferation in pancreatic cancer cells via regulation of the c-Myc-p21Waf1 signalling pathway.
Du S, Wang H, Cai J, Ren R, Zhang W, Wei W, Shen X
Biochemistry and cell biology = Biochimie et biologie cellulaire 2020 Apr;98(2):191-202
Biochemistry and cell biology = Biochimie et biologie cellulaire 2020 Apr;98(2):191-202
Long noncoding RNA FBXL19-AS1 induces tumor growth and metastasis by sponging miR-203a-3p in lung adenocarcinoma.
Wang L, Zhang X, Liu Y, Xu S
Journal of cellular physiology 2020 Apr;235(4):3612-3625
Journal of cellular physiology 2020 Apr;235(4):3612-3625
S-Adenosylmethionine Affects Cell Cycle Pathways and Suppresses Proliferation in Liver Cells.
Yan L, Liang X, Huang H, Zhang G, Liu T, Zhang J, Chen Z, Zhang Z, Chen Y
Journal of Cancer 2019;10(18):4368-4379
Journal of Cancer 2019;10(18):4368-4379
Bromodomain inhibitor jq1 induces cell cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway.
Wen N, Guo B, Zheng H, Xu L, Liang H, Wang Q, Wang D, Chen X, Zhang S, Li Y, Zhang L
International journal of oncology 2019 Oct;55(4):879-895
International journal of oncology 2019 Oct;55(4):879-895
Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.
Su D, Zhang D, Jin J, Ying L, Han M, Chen K, Li B, Wu J, Xie Z, Zhang F, Lin Y, Cheng G, Li JY, Huang M, Wang J, Wang K, Zhang J, Li F, Xiong L, Futreal A, Mao W
Nature communications 2019 Nov 7;10(1):5076
Nature communications 2019 Nov 7;10(1):5076
Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway.
Jiang X, Wang J
Journal of cellular and molecular medicine 2019 Jul;23(7):4545-4558
Journal of cellular and molecular medicine 2019 Jul;23(7):4545-4558
Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.
Li Y, Kong R, Chen H, Zhao Z, Li L, Li J, Hu J, Zhang G, Pan S, Wang Y, Wang G, Chen H, Sun B
Aging 2019 Jul 21;11(14):5035-5057
Aging 2019 Jul 21;11(14):5035-5057
Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.
Li T, Xiong Y, Wang Q, Chen F, Zeng Y, Yu X, Wang Y, Zhou F, Zhou Y
Artificial cells, nanomedicine, and biotechnology 2019 Dec;47(1):4001-4011
Artificial cells, nanomedicine, and biotechnology 2019 Dec;47(1):4001-4011
Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.
Xiong Y, Li T, Assani G, Ling H, Zhou Q, Zeng Y, Zhou F, Zhou Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Apr;112:108602
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Apr;112:108602
Roles of B7-H3 in Cervical Cancer and Its Prognostic Value.
Han S, Shi X, Liu L, Zong L, Zhang J, Chen Q, Qian Q, Chen L, Wang Y, Jin J, Ma Y, Cui B, Yang X, Zhang Y
Journal of Cancer 2018;9(15):2612-2624
Journal of Cancer 2018;9(15):2612-2624
Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy.
Zhu D, Shen H, Tan S, Hu Z, Wang L, Yu L, Tian X, Ding W, Ren C, Gao C, Cheng J, Deng M, Liu R, Hu J, Xi L, Wu P, Zhang Z, Ma D, Wang H
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Oct 3;26(10):2443-2455
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Oct 3;26(10):2443-2455
Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.
Chen X, Liu C, Zhao R, Zhao P, Wu J, Zhou N, Ying M
Molecular pharmaceutics 2018 Nov 5;15(11):4974-4984
Molecular pharmaceutics 2018 Nov 5;15(11):4974-4984
Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.
Yun DP, Wang YQ, Meng DL, Ji YY, Chen JX, Chen HY, Lu DR
Oncology reports 2018 Mar;39(3):1011-1022
Oncology reports 2018 Mar;39(3):1011-1022
TACC3 transcriptionally upregulates E2F1 to promote cell growth and confer sensitivity to cisplatin in bladder cancer.
Lin ZR, Wang MY, He SY, Cai ZM, Huang WR
Cell death & disease 2018 Jan 22;9(2):72
Cell death & disease 2018 Jan 22;9(2):72
Whole exome-wide association study identifies a missense variant in SLC2A4RG associated with glioblastoma risk.
Zhao Y, Yun D, Zou X, Jiang T, Li G, Hu L, Chen J, Xu J, Mao Y, Chen H, Lu D
American journal of cancer research 2017;7(9):1937-1947
American journal of cancer research 2017;7(9):1937-1947
Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica.
Zhang S, Yao F, Jing T, Zhang M, Zhao W, Zou X, Sui L, Hou L
Gene 2017 Sep 10;628:170-179
Gene 2017 Sep 10;628:170-179
The transcription factor FOXA1 induces epithelial ovarian cancer tumorigenesis and progression.
Wang LL, Xiu YL, Chen X, Sun KX, Chen S, Wu DD, Liu BL, Zhao Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 May;39(5):1010428317706210
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 May;39(5):1010428317706210
AhR‑E2F1‑KGFR signaling is involved in KGF‑induced intestinal epithelial cell proliferation.
Yang K, Yin J, Sheng B, Wang Q, Han B, Pu A, Yu M, Sun L, Xiao W, Yang H
Molecular medicine reports 2017 May;15(5):3019-3026
Molecular medicine reports 2017 May;15(5):3019-3026
RACK1 promotes lung cancer cell growth via an MCM7/RACK1/ Akt signaling complex.
Fei L, Ma Y, Zhang M, Liu X, Luo Y, Wang C, Zhang H, Zhang W, Han Y
Oncotarget 2017 Jun 20;8(25):40501-40513
Oncotarget 2017 Jun 20;8(25):40501-40513
In vitro and ex vivo delivery of tailored siRNA-nanoliposomes for E2F1 silencing as a potential therapy for colorectal cancer.
Bochicchio S, Dapas B, Russo I, Ciacci C, Piazza O, De Smedt S, Pottie E, Barba AA, Grassi G
International journal of pharmaceutics 2017 Jun 20;525(2):377-387
International journal of pharmaceutics 2017 Jun 20;525(2):377-387
Roles of immune inhibitory molecule B7-H4 in cervical cancer.
Han S, Li Y, Zhang J, Liu L, Chen Q, Qian Q, Li S, Zhang Y
Oncology reports 2017 Apr;37(4):2308-2316
Oncology reports 2017 Apr;37(4):2308-2316
Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.
Zhang M, Yao F, Qin T, Hou L, Zou X
Gene 2017 Apr 15;608:28-40
Gene 2017 Apr 15;608:28-40
Dihydroartemisinin suppresses pancreatic cancer cells via a microRNA-mRNA regulatory network.
Li Y, Wang Y, Kong R, Xue D, Pan S, Chen H, Sun B
Oncotarget 2016 Sep 20;7(38):62460-62473
Oncotarget 2016 Sep 20;7(38):62460-62473
SUMOylation of Rb enhances its binding with CDK2 and phosphorylation at early G1 phase.
Meng F, Qian J, Yue H, Li X, Xue K
Cell cycle (Georgetown, Tex.) 2016 Jul 2;15(13):1724-32
Cell cycle (Georgetown, Tex.) 2016 Jul 2;15(13):1724-32
Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1).
Lu Z, Xiao Z, Liu F, Cui M, Li W, Yang Z, Li J, Ye L, Zhang X
Oncotarget 2016 Jan 5;7(1):241-54
Oncotarget 2016 Jan 5;7(1):241-54
CIP2A is associated with multidrug resistance in cervical adenocarcinoma by a P-glycoprotein pathway.
Liu J, Wang M, Zhang X, Wang Q, Qi M, Hu J, Zhou Z, Zhang C, Zhang W, Zhao W, Wang X
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Feb;37(2):2673-82
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Feb;37(2):2673-82
miR-148a-3p represses proliferation and EMT by establishing regulatory circuits between ERBB3/AKT2/c-myc and DNMT1 in bladder cancer.
Wang X, Liang Z, Xu X, Li J, Zhu Y, Meng S, Li S, Wang S, Xie B, Ji A, Liu B, Zheng X, Xie L
Cell death & disease 2016 Dec 1;7(12):e2503
Cell death & disease 2016 Dec 1;7(12):e2503
Regulation of Hepatic Cholesteryl Ester Transfer Protein Expression and Reverse Cholesterol Transport by Inhibition of DNA Topoisomerase II.
Liu M, Chen Y, Zhang L, Wang Q, Ma X, Li X, Xiang R, Zhu Y, Qin S, Yu Y, Jiang XC, Duan Y, Han J
The Journal of biological chemistry 2015 Jun 5;290(23):14418-29
The Journal of biological chemistry 2015 Jun 5;290(23):14418-29
TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.
Hu Z, Ding W, Zhu D, Yu L, Jiang X, Wang X, Zhang C, Wang L, Ji T, Liu D, He D, Xia X, Zhu T, Wei J, Wu P, Wang C, Xi L, Gao Q, Chen G, Liu R, Li K, Li S, Wang S, Zhou J, Ma D, Wang H
The Journal of clinical investigation 2015 Jan;125(1):425-36
The Journal of clinical investigation 2015 Jan;125(1):425-36
The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.
Wang Y, Cui M, Cai X, Sun B, Liu F, Zhang X, Ye L
Cancer letters 2014 Oct 1;352(2):169-78
Cancer letters 2014 Oct 1;352(2):169-78
Notch1 is a potential therapeutic target for the treatment of human hepatitis B virus X protein-associated hepatocellular carcinoma.
Sun Q, Wang R, Wang Y, Luo J, Wang P, Cheng B
Oncology reports 2014 Feb;31(2):933-9
Oncology reports 2014 Feb;31(2):933-9
Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.
Valle BL, D'Souza T, Becker KG, Wood WH 3rd, Zhang Y, Wersto RP, Morin PJ
PloS one 2013;8(4):e61836
PloS one 2013;8(4):e61836
Hepatitis B virus X protein inhibits tumor suppressor miR-205 through inducing hypermethylation of miR-205 promoter to enhance carcinogenesis.
Zhang T, Zhang J, Cui M, Liu F, You X, Du Y, Gao Y, Zhang S, Lu Z, Ye L, Zhang X
Neoplasia (New York, N.Y.) 2013 Nov;15(11):1282-91
Neoplasia (New York, N.Y.) 2013 Nov;15(11):1282-91
The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.
Xu F, You X, Liu F, Shen X, Yao Y, Ye L, Zhang X
Cancer letters 2013 Jun 1;333(1):124-32
Cancer letters 2013 Jun 1;333(1):124-32
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP(E2F1 antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The E2F1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human E2F1. This antibody recognizes human,mouse,rat antigen. The E2F1 antibody has been validated for the following applications: ELISA, IHC, WB analysis.
