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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX112912 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112912, RRID:AB_1950158
- Product name
- E2F1 antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references RB maintains quiescence and prevents premature senescence through upregulation of DNMT1 in mesenchymal stromal cells.
Lin SP, Chiu FY, Wang Y, Yen ML, Kao SY, Hung SC
Stem cell reports 2014 Dec 9;3(6):975-86
Stem cell reports 2014 Dec 9;3(6):975-86
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX112912 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 10% SDS PAGE GTX112912 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- E2F1 antibody [N2C2], Internal detects E2F1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E2F1 protein stained by E2F1 antibody [N2C2], Internal (GTX112912) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.
