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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- TA346935 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal E2F-1 Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal E2F-1 Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- E2F1
- Isotype
- IgG
- Antibody clone number
- 4G8-2C2-C12
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.
- Validation comment
- IP
