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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [1]
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- Product number
- PAB1637 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1637, RRID:AB_1238051
- Product name
- E2F1 (phospho S332) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of E2F1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors.
c-Myc-regulated microRNAs modulate E2F1 expression.
Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism.
Joshi B, Ordonez-Ercan D, Dasgupta P, Chellappan S
Oncogene 2005 Mar 24;24(13):2204-17
Oncogene 2005 Mar 24;24(13):2204-17
c-Myc-regulated microRNAs modulate E2F1 expression.
O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT
Nature 2005 Jun 9;435(7043):839-43
Nature 2005 Jun 9;435(7043):839-43
Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism.
Wang C, Hou X, Mohapatra S, Ma Y, Cress WD, Pledger WJ, Chen J
The Journal of biological chemistry 2005 Apr 1;280(13):12339-43
The Journal of biological chemistry 2005 Apr 1;280(13):12339-43
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Dot blot analysis of E2F1 (phospho S332) polyclonal antibody (Cat # PAB1637) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
