M00257

antibody from Boster Biological Technology

Targeting: E2F1 RBBP3, RBP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00257

Provider

Boster Biological Technology

Product name

Anti-E2F1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for E2F-1/E2F1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat E2F-1/E2F1 information: Molecular Weight: 46920 MW; Subcellular Localization: Nucleus.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

IGD-5

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

U2 small nuclear RNA auxiliary factor 2, transcriptionally activated by the transcription factor Dp-1/E2F transcription factor 1 complex, enhances the growth and aerobic glycolysis of leiomyosarcoma cells.

Li Y, Chen S, Zhang X, Zhuo N
Bioengineered 2022 Apr;13(4):10200-10212

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Regulating A549 cells growth by ASO inhibiting miRNA expression.

Wang PY, Li YJ, Zhang S, Li ZL, Yue Z, Xie N, Xie SY
Molecular and cellular biochemistry 2010 Jun;339(1-2):163-71

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Interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma.

Wang X, Huang K, Xu L
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2009 Dec;29(6):729-31

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of E2F1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (M00257). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F1 monoclonal antibody (Catalog # M00257) overnight at 4

Additional image