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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358119 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-E2F Transcription Factor 1 (E2F1) (pSer337) antibody
- Antibody type
- Polyclonal
- Description
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer337
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors.
c-Myc-regulated microRNAs modulate E2F1 expression.
Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism.
Transcriptional regulation of AKT activation by E2F.
Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow.
Joshi B, Ordonez-Ercan D, Dasgupta P, Chellappan S
Oncogene 2005 Mar 24;24(13):2204-17
Oncogene 2005 Mar 24;24(13):2204-17
c-Myc-regulated microRNAs modulate E2F1 expression.
O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT
Nature 2005 Jun 9;435(7043):839-43
Nature 2005 Jun 9;435(7043):839-43
Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism.
Wang C, Hou X, Mohapatra S, Ma Y, Cress WD, Pledger WJ, Chen J
The Journal of biological chemistry 2005 Apr 1;280(13):12339-43
The Journal of biological chemistry 2005 Apr 1;280(13):12339-43
Transcriptional regulation of AKT activation by E2F.
Chaussepied M, Ginsberg D
Molecular cell 2004 Dec 3;16(5):831-7
Molecular cell 2004 Dec 3;16(5):831-7
Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow.
Saberwal G, Lucas S, Janssen I, Deobhakta A, Hu WY, Galili N, Raza A, Mundle SD
International journal of hematology 2004 Aug;80(2):146-54
International journal of hematology 2004 Aug;80(2):146-54
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