MA5-14344

antibody from Invitrogen Antibodies

Targeting: E2F1 RBBP3, RBP3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Gel shift (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14344

Provider

Invitrogen Antibodies

Product name

E2F1 Monoclonal Antibody (KH95)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-14344 targets E2F-1 Transcription Factor in gel shift, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human and mouse samples. The MA5-14344 immunogen is recombinant human E2F-1 protein.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

KH95

Vial size

500 µL

Concentration

0.4 mg/mL

Storage

4° C

References

Submitted references

Anti-cancer binary system activated by bacteriophage HK022 integrase.

Elias A, Gritsenko N, Gorovits R, Spector I, Prag G, Yagil E, Kolot M
Oncotarget 2018 Jun 8;9(44):27487-27501

---

Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease.

Silva AR, Santos AC, Farfel JM, Grinberg LT, Ferretti RE, Campos AH, Cunha IW, Begnami MD, Rocha RM, Carraro DM, de Bragança Pereira CA, Jacob-Filho W, Brentani H
PloS one 2014;9(6):e99897

---

The relationship between E2F family members and tumor growth in colorectal adenocarcinomas: A comparative immunohistochemical study of 100 cases.

Xanthoulis A, Kotsinas A, Tiniakos D, Fiska A, Tentes AA, Kyroudi A, Kittas C, Gorgoulis V
Applied immunohistochemistry & molecular morphology : AIMM 2014 Jul;22(6):471-7

---

CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy.

McClendon AK, Dean JL, Rivadeneira DB, Yu JE, Reed CA, Gao E, Farber JL, Force T, Koch WJ, Knudsen ES
Cell cycle (Georgetown, Tex.) 2012 Jul 15;11(14):2747-55

---

Identification of prognostic biomarkers for glioblastomas using protein expression profiling.

Jung Y, Joo KM, Seong DH, Choi YL, Kong DS, Kim Y, Kim MH, Jin J, Suh YL, Seol HJ, Shin CS, Lee JI, Kim JH, Song SY, Nam DH
International journal of oncology 2012 Apr;40(4):1122-32

---

Enhanced expression of 70-kilodalton heat shock protein limits cell division in a sepsis-induced model of acute respiratory distress syndrome.

Bromberg Z, Raj N, Goloubinoff P, Deutschman CS, Weiss YG
Critical care medicine 2008 Jan;36(1):246-55

---

Impact of E2F-1 expression on clinical outcome of gastric adenocarcinoma patients with adjuvant chemoradiation therapy.

Lee J, Park CK, Park JO, Lim T, Park YS, Lim HY, Lee I, Sohn TS, Noh JH, Heo JS, Kim S, Lim DH, Kim KM, Kang WK
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jan 1;14(1):82-8

---

Deregulated expression of p16INK4a and p53 pathway members in benign and malignant myoepithelial tumours of the salivary glands.

Vékony H, Röser K, Löning T, Raaphorst FM, Leemans CR, Van der Waal I, Bloemena E
Histopathology 2008 Dec;53(6):658-66

---

Overexpression of N-Myc rapidly causes acute myeloid leukemia in mice.

Kawagoe H, Kandilci A, Kranenburg TA, Grosveld GC
Cancer research 2007 Nov 15;67(22):10677-85

---

Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.

Au NH, Cheang M, Huntsman DG, Yorida E, Coldman A, Elliott WM, Bebb G, Flint J, English J, Gilks CB, Grimes HL
The Journal of pathology 2004 Sep;204(1):101-9

---

Expression of E2F-4 gene in colorectal adenocarcinoma and corresponding covering mucosa: an immunohistochemistry, image analysis, and immunoblot study.

Mady HH, Hasso S, Melhem MF
Applied immunohistochemistry & molecular morphology : AIMM 2002 Sep;10(3):225-30

---

UCN-01 suppresses E2F-1 mediated by ubiquitin-proteasome-dependent degradation.

Hsueh CT, Wu YC, Schwartz GK
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Mar;7(3):669-74

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of E2F-1 Transcription Factor using E2F-1 Transcription Factor Monoclonal Antibody (Product # MA5-14344) on MAD109 Cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with E2F-1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of the cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of E2F-1 Transcription Factor (KH95) showing staining in the nucleus of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E2F-1 Transcription Factor Antibody (KH95) Mouse Monoclonal Antibody (Product # MA5-14344) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of E2F-1 Transcription Factor using E2F-1 Transcription Factor Monoclonal Antibody (Product # MA5-14344) on Native Mouse MAD109 Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of E2F-1 Transcription Factor using E2F-1 Transcription Factor Monoclonal Antibody (Product # MA5-14344) on denatured Mouse MAD109 Cells.