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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB600-900 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-900, RRID:AB_10002971
- Product name
- Rabbit Polyclonal E2F-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. E2F1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - NB600-900 Western blot - E2F1 (phospho S364) antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Analysis of a band at 47 kDa corresponding to phosphorylated E2F-1 in induced cell lysates. Panel A shows reactivity using a control antibody reactive to all forms of E2F (arrowheads). Panel B shows specific reactivity against phosphorylated E2F-1 (arrowheads) using our anti-E2F-1 pS364 antibody. Lysates are as follows: CRE/E2F-1 are CRE cells derived from mouse NIH3T3 line transfected with human E2F-1, NIH-3T3 used as a negative control, and MDA-incubated with blocking buffer for 45 min at room temperature for ECL detection.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: E2F-1 [p Ser364] Antibody [NB600-900] - Used at a 10 ug/ml to detect nuclear and occasion-ally cytoplasmic signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear staining between individual tumors, with some tumors showing strong staining. This image shows E2F-1 pS364 staining of human breast carcinoma. Tissue was formalin-fixed and paraffin embedded.
