Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-56080 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56080, RRID:AB_837662
- Product name
- Rabbit Polyclonal Bad Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Bad
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bad Antibody [NB100-56080] - Analysis of Bad in Daoy whole cell lysate using anti-Bad antibody. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bad Antibody [NB100-56080] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using 1:2000 conc. of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Bad was observed. Staining was performed by Histowiz.