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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX101249 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101249, RRID:AB_1240560
- Product name
- CBP antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: H1299 5% SDS PAGE GTX101249 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and CBP knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and CBP knockout (KO) HeLa cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP (GTX101249) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CBP antibody [C3], C-term detects CBP protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CBP protein stained by CBP antibody [C3], C-term (GTX101249) diluted at 1:1000.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- CBP antibody immunoprecipitates CBP protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of CBP protein by 2.5 £gg of CBP antibody (GTX101249) 12% SDS-PAGE The immunoprecipitated CBP protein was detected by CBP antibody (GTX101249) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CBP antibody [C3], C-term (GTX101249) dilution: 1:750.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CBP antibody [C3], C-term (GTX101249) dilution: 1:750.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CBP antibody [C3], C-term (GTX101249) dilution: 1:750.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. CBP antibody [C3], C-term (GTX101249) dilution: 1:750.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CBP antibody [C3], C-term (GTX101249) dilution: 1:750.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 £gg preimmune rabbit IgG B. 5 £gg of CREBBP antibody (GTX101249) The precipitated DNA was detected by PCR with primer set targeting to snail promoter.
