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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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- Product number
- NB100-382 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-382, RRID:AB_10001808
- Product name
- Rabbit Polyclonal CBP/KAT3A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Tamoxifen Resistance in Breast Cancer Is Regulated by the EZH2-ERĪ±-GREB1 Transcriptional Axis.
Wu Y, Zhang Z, Cenciarini ME, Proietti CJ, Amasino M, Hong T, Yang M, Liao Y, Chiang HC, Kaklamani VG, Jeselsohn R, Vadlamudi RK, Huang TH, Li R, De Angelis C, Fu X, Elizalde PV, Schiff R, Brown M, Xu K
Cancer research 2018 Feb 1;78(3):671-684
Cancer research 2018 Feb 1;78(3):671-684
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CBP/KAT3A Antibody [NB100-382] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: Affinity purified rabbit anti-CBP used at 20 ug/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CBP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Detection of human CBP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-381. For blotting immunoprecipitated CBP, NB100-382 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - FFPE section of human prostate adenocarcinoma. Antibody used at a dilution of 1:250. Detection: DAB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBP used at a dilution of 1:500 (2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
