sc-28327

antibody from Santa Cruz Biotechnology

Targeting: EWSR1 EWS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28327

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28327, RRID:AB_675526

Product name

Anti-EWSR1

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 38

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.