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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- R36298 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EWS Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific EWS antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the EWS antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of nuclear HeLa lysate with EWS antibody at 0.3ug/ml. Predicted molecular weight: ~69kDa, but routinely observed at ~90kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of nuclear HeLa lysate with EWS antibody at 0.3ug/ml. Predicted molecular weight: ~69kDa, but routinely observed at ~90kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney tissue with EWS antibody at 7ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Negative control: IHC staining of FFPE human kidney tissue without primary antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
