Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-77025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EWSR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-EWSR1 Polyclonal Antibody (Product # PA5-77025) and 68, 61 kDa bands corresponding to EWSR1 was observed across the cell lines and tissue tested along with uncharacterized bands (*). Modified whole cell extracts (1% SDS) (30 µg lysate) of MOLT-4 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), HEK-293 (Lane 4), NIH/3T3 (Lane 5), MCF7 (Lane 6), RAW 264.7 (Lane 7), Jurkat (Lane 8), Mouse Uterus (Lane 9) and Rat Testis (Lane 10) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of EWSR1 in HeLa cells. Samples were incubated with EWSR1 polyclonal antibody (Product # PA5-77025).