Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-19311 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Ku70/XRCC6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ku70 Antibody [NBP2-19311] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ku70/XRCC6 Antibody [NBP2-19311] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ku70 Antibody [NBP2-19311] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2. 5 ug of Ku70 antibody 7. 5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ku70 Antibody [NBP2-19311] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody [NBP2-19311] - Human cervical cancer. Ku70 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
