10723-1-AP

antibody from Proteintech Group

Targeting: XRCC6 D22S671, D22S731, G22P1, KU70, ML8

Provider product page for 10723-1-AP

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Immunocytochemistry

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Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [50]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 10723-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10723-1-AP, RRID:AB_2218756
Product name: KU70 antibody
Antibody type: Polyclonal
Description: KD/KO validated KU70 antibody (Cat. #10723-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

XRCC6 protein structure - 10723-1-AP shown in red.

Submitted references O-GlcNAcylation of NONO mediates alternative splicing of SETMAR and facilitates NHEJ repair.

Li M, Tian H, Zhou Z, Jiang Y, Guo X, Qin W, Zhang H, Jiao Y, Guo S, Wu C
Genome biology 2026 Jan 14;27(1):26

FOXN3 integrates the KU70/KU80/SREBP-1 complex to regulate lipid metabolism in non-alcoholic fatty liver disease.

Du J, Jin L, Wang S, Hu X, Yu J, Yu J, Huo Q, Wu N, Liu X, Yang Y, Zhang Y, Huang S, Zhou J, Zheng SG, Zheng C, Zhu X
Nucleic acids research 2026 Feb 24;54(5)

Xrcc6 coordinates cardiomyocyte repair and immune regulation in myocardial ischemia-reperfusion injury: Fisetin as a therapeutic modulator.

He Y, Li J, Guo L, Chen M, Pan H, Li Z, Tian H, Yu H, Zhou Y, Xiao H
Frontiers in immunology 2025;16:1653738

Targeting CLK1/SRSF7 axis-dependent alternative splicing sensitizes pancreatic ductal adenocarcinoma to chemotherapy and immunotherapy.

Zhang C, Chen Y, Zhu S, Wang Z, Lin H, Lu J, Zhang H, Weng Y, Huang X, Li G, Wu Y, Li Z, Hu J, Xie C, Lai J, Tian Y, Liao C, Chen S
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2025 Nov;83:101292

KAT2A-driven succinylation of SRSF11 enforces spliceosome-mediated RAD52 splicing to promote homologous recombination and radioresistance in hepatocellular carcinoma.

Wu J, Yuan J, Liu Z, Zhou Y, Zhang B, Xu Y, Zeng Q, Wu Z, Kong L, Wang J, Zhang B, Yang J, Lv T, Shi Y, Yang J
Signal transduction and targeted therapy 2025 Nov 7;10(1):364

CNOT7 facilitates radiation resistance in colorectal cancer through TRIM21/XRCC6-mediated non-homologous end joining repair.

Li Y, Cui L, Wang S, Zhao Q, Du F, Du S, Yu C, Xia M, Zhao S, Luan T, Liu Y, Cui B
Cell death & disease 2025 Nov 17;16(1):833

GX15-070 enhances niraparib efficacy in ovarian cancer by promoting a shift in Mcl1-mediated DNA repair pathway from HR to NHEJ.

Sheng JJ, He Y, Liu PW, Zheng SA, Wu N, Sui HY, Cheng Q, Li H
Journal of translational medicine 2025 Nov 11;23(1):1262

Nuclear-Localized BCKDK Facilitates Homologous Recombination Repair to Support Breast Cancer Progression and Therapy Resistance.

Liu H, Feng J, Pan T, Zhang P, Ye L, Jiang Z, Zhou Z, Mao Q, Li J, Yang X, Gao P, Huang D, Zhang H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jun;12(22):e2416590

Nuclear VPS35 attenuates NHEJ repair by sequestering Ku protein.

Zhang L, Nie Y, Tang T, Lu Y, Li W, Hong X, Li Q, Zheng A, Li Y, Zhou J, Fan L, Wang T, Deng Z
Molecular medicine (Cambridge, Mass.) 2025 Jun 9;31(1):222

Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.

He J, Lai T, Zhou Z, Yang H, Lei Z, Zhou L, Li N, He Y, Zeng S, Munai E, Tan Y, Wang M, Zhang Y, Zhou W, Wu Y
Scientific reports 2025 Jul 9;15(1):24651

Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway.

Ding X, Wang Y, Shao Z, Wen X, Wang B, Liu G, Xu C, Chai L, Qiu H, Zhang L
Discover oncology 2025 Jul 31;16(1):1451

Chromatin Perturbation Promotes Susceptibility to Hypomethylating Agents.

Schneider C, Alexe G, Merickel LA, Meyer A, Swift ML, Serafim RB, Basanthakumar AT, Taillon A, Salhotra S, Boniolo F, Lambo S, Rodriguez Y, Häupl B, George RE, Root DE, Oellerich T, Hovestadt V, Chowdhury D, Stegmaier K
bioRxiv : the preprint server for biology 2025 Jul 31;

Mutant p53 upregulates HDAC6 to resist ER stress and facilitates Ku70 deacetylation, which prevents its degradation and mitigates DNA damage in colon cancer cells.

Benedetti R, Di Crosta M, Gilardini Montani MS, D'Orazi G, Cirone M
Cell death discovery 2025 Apr 10;11(1):162

Synergistic Effect of Ubiquitin-Specific Protease 14 and Poly(ADP-Ribose) Glycohydrolase Co-Inhibition in BRCA1-Mutant, Poly(ADP-Ribose) Polymerase Inhibitor-Resistant Triple-Negative Breast Cancer Cells.

Li P, Zhu X, Qu H, Han Z, Yao X, Wei Y, Li B, Chen H
OncoTargets and therapy 2024;17:741-753

Nonclassical action of Ku70 promotes Treg-suppressive function through a FOXP3-dependent mechanism in lung adenocarcinoma.

Huang Q, Tian N, Zhang J, Song S, Cheng H, Liu X, Zhang W, Ye Y, Du Y, Dai X, Liang R, Li D, Dai SM, Wang C, Chen Z, Zhou Q, Li B
The Journal of clinical investigation 2024 Oct 24;134(23)

Glycyrrhizin ameliorates colorectal cancer progression by regulating NHEJ pathway through inhibiting HMGB1-induced DNA damage response.

Han Y, Sheng W, Liu X, Liu H, Jia X, Li H, Wang C, Wang B, Hu T, Ma Y
Scientific reports 2024 Oct 23;14(1):24948

Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability.

Harper KL, Harrington EM, Hayward C, Anene CA, Wongwiwat W, White RE, Whitehouse A
Nature communications 2024 Nov 26;15(1):10240

DNA-PK-Mediated Cytoplasmic DNA Sensing Stimulates Glycolysis to Promote Lung Squamous Cell Carcinoma Malignancy and Chemoresistance.

Wang H, Zhang Y, Tian Y, Yang W, Wang Y, Hou H, Pan H, Pei S, Zhu H, Gu Z, Zhang Y, Dai D, Chen W, Zheng M, Luo Q, Xiao Y, Huang J
Cancer research 2024 Mar 4;84(5):688-702

MicroRNA-384 radiosensitizes human non-small cell lung cancer by impairing DNA damage response and repair signaling, which is inhibited by NF-κB.

Sun Y, Wang J, Qiu M, Zhao J, Zou F, Meng M, Jiang X, Yuan Z, Mi Z, Wu Z
Cancer biology & medicine 2024 Jun 27;21(11):1050-66

CircUGGT2 facilitates progression and cisplatin resistance of bladder cancer through nonhomologous end-joining pathway.

Lyu F, Huang S, Yan Z, He Q, Liu C, Cheng L, Cong Y, Chen K, Song Y, Xing Y
Cellular signalling 2024 Jul;119:111164

FAM21 interacts with Ku to promote the localization of WASH to DNA double strand break sites.

Wang T, Zheng AX, Li P, Tang T, Zhang LP, Hong Y, Hong X, Deng ZH
DNA repair 2024 Jan;133:103603

Ku70 silencing aggravates oxygen-glucose deprivation/reperfusion-induced injury by activation of the p53 apoptotic pathway in rat cortical astrocytes.

Xie X, Liu J
Histochemistry and cell biology 2024 Dec 23;163(1):20

BCAT1 Associates with DNA Repair Proteins KU70 and KU80 and Contributes to Regulate DNA Repair in T-Cell Acute Lymphoblastic Leukemia (T-ALL).

Tosello V, Rompietti C, Papathanassiu AE, Arrigoni G, Piovan E
International journal of molecular sciences 2024 Dec 18;25(24)

LINC-PINT plays an anti-tumor role in nasopharyngeal carcinoma by binding to XRCC6 and affecting its function.

Guo Z, Guan K, Bao M, He B, Lu J
Pathology, research and practice 2024 Aug;260:155460

CircNOP14 increases the radiosensitivity of hepatocellular carcinoma via inhibition of Ku70-dependent DNA damage repair.

Lin L, Hu P, Luo M, Chen X, Xiao M, Zhong Z, Peng S, Chen G, Yang G, Zhang F, Zhang Y
International journal of biological macromolecules 2024 Apr;264(Pt 2):130541

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis.

Bao K, Ma Y, Li Y, Shen X, Zhao J, Tian S, Zhang C, Liang C, Zhao Z, Yang Y, Zhang K, Yang N, Meng FL, Hao J, Yang J, Liu T, Yao Z, Ai D, Shi L
Molecular cell 2024 Apr 4;84(7):1206-1223.e15

C9orf72 functions in the nucleus to regulate DNA damage repair.

He L, Liang J, Chen C, Chen J, Shen Y, Sun S, Li L
Cell death and differentiation 2023 Mar;30(3):716-730

LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer.

Dey G, Bharti R, Braley C, Alluri R, Esakov E, Crean-Tate K, McCrae K, Joehlin-Price A, Rose PG, Lathia J, Gong Z, Reizes O
Journal of ovarian research 2023 Jun 27;16(1):122

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy.

Wei Y, Xiao G, Xu H, Sun X, Shi Y, Wang F, Kang J, Peng J, Zhou F
The FEBS journal 2023 Jul;290(14):3629-3645

Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer.

Chen M, Zhao E, Li M, Xu M, Hao S, Gao Y, Wu X, Li X, Yu Y, Yu Z, Yin Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jul 25;116:154876

Screening and characterization of myositis-related autoantibodies in COVID-19 patients.

Teo KF, Chen DY, Hsu JT, Lai YH, Chang CK, Hsueh PR, Lan JL, Hsu JL
Clinical and translational science 2023 Jan;16(1):140-150

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway.

Wen X, Chen S, Chen X, Qiu H, Wang W, Zhang N, Liu W, Wang T, Ding X, Zhang L
Frontiers in oncology 2022;12:887068

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells.

Li Q, Yao H, Wang Y, Wu Y, Thorne RF, Zhu Y, Wu M, Liu L
Cell reports 2022 Nov 22;41(8):111707

NRBF2 regulates the chemoresistance of small cell lung cancer by interacting with the P62 protein in the autophagy process.

Shen W, Luo P, Sun Y, Zhang W, Zhou N, Zhan H, Zhang Q, Shen J, Lin A, Cheng Q, Wang Q, Zhang J, Wang HH, Wei T
iScience 2022 Jun 17;25(6):104471

WASH interacts with Ku to regulate DNA double-stranded break repair.

Wang T, Du XH, Hong Y, Hong X, Fan L, Zhou JW, Sun H, Ge J, Billadeau DD, Deng ZH
iScience 2022 Jan 21;25(1):103676

Proteomic Analysis of Vero Cells Infected with Pseudorabies Virus.

Yang X, Xu S, Chen D, Jiang R, Kang H, Ge X, Zhou L, Han J, Zhang Y, Guo X, Yang H
Viruses 2022 Apr 4;14(4)

Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability.

Cao C, Han P, Liu L, Tang Y, Tian S, Zhang K, Shi L, Liu Z, Zhuo D, Ge W, Gong W
The Journal of biological chemistry 2021 Sep;297(3):101036

Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca(2+)](m)-PDP1-PDH-histone acetylation retrograde signaling.

Shi Y, Wang Y, Jiang H, Sun X, Xu H, Wei X, Wei Y, Xiao G, Song Z, Zhou F
Cell death & disease 2021 Sep 6;12(9):837

Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer.

Li S, Sun W, Luo Y, Gao Y, Jiang X, Yuan C, Han L, Cao K, Gong Y, Xie C
Journal of materials chemistry. B 2021 Jun 16;9(23):4643-4653

OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.

Cui Y, Xie R, Zhang X, Liu Y, Hu Y, Li Y, Liu X, Yu X, Wu C
Cell death & disease 2021 Jun 16;12(7):622

LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma.

Guo Z, Wang YH, Xu H, Yuan CS, Zhou HH, Huang WH, Wang H, Zhang W
Cell death & disease 2021 Jan 4;12(1):69

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Li G, Zhang X, Wang H, Liu D, Li Z, Wu Z, Yang H
The international journal of biochemistry & cell biology 2020 Aug;125:105790

Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.

Aiken CE, Tarry-Adkins JL, Spiroski AM, Nuzzo AM, Ashmore TJ, Rolfo A, Sutherland MJ, Camm EJ, Giussani DA, Ozanne SE
The Journal of physiology 2019 May;597(9):2391-2401

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

Aiken CE, Tarry-Adkins JL, Spiroski AM, Nuzzo AM, Ashmore TJ, Rolfo A, Sutherland MJ, Camm EJ, Giussani DA, Ozanne SE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jun;33(6):7758-7766

Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Wang Y, Hu L, Zhang X, Zhao H, Xu H, Wei Y, Jiang H, Xie C, Zhou Y, Zhou F
Journal of Cancer 2017;8(8):1400-1409

USP22 Induces Cisplatin Resistance in Lung Adenocarcinoma by Regulating γH2AX-Mediated DNA Damage Repair and Ku70/Bax-Mediated Apoptosis.

Wang A, Ning Z, Lu C, Gao W, Liang J, Yan Q, Tan G, Liu J
Frontiers in pharmacology 2017;8:274

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Hu L, Li X, Liu Q, Xu J, Ge H, Wang Z, Wang H, Wang Z, Shi C, Xu X, Huang J, Lin Z, Pieper RO, Weng C
Oncogene 2017 Feb 23;36(8):1145-1156

High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the β-Catenin/Wnt Signaling Pathway and Is Associated with Poor Prognosis.

Zhu B, Cheng D, Li S, Zhou S, Yang Q
International journal of molecular sciences 2016 Jul 22;17(7)

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Dong H, Shi Q, Song X, Fu J, Hu L, Xu D, Su C, Xia X, Song E, Song Y
Toxicology and applied pharmacology 2015 Jul 1;286(1):10-6

RNAi silencing targeting RNF8 enhances radiosensitivity of a non-small cell lung cancer cell line A549.

Zhou H, Mu X, Chen J, Liu H, Shi W, Xing E, Yang K, Wu G
International journal of radiation biology 2013 Sep;89(9):708-15

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10723-1-AP(KU70,XRCC6 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 10723-1-AP(KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded rat testis using 10723-1-AP(KU70,XRCC6 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue