Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [2]
 - Immunocytochemistry [2]
 - Immunohistochemistry [1]
 - Flow cytometry [1]
 
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Validation data
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- Product number
 - F49015 - Provider product page

 - Provider
 - NSJ Bioreagents
 - Product name
 - XRCC6 Antibody
 - Antibody type
 - Polyclonal
 - Description
 - This highly specific XRCC6 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence/ELISA applications with human samples.
 - Reactivity
 - Human
 - Host
 - Rabbit
 - Conjugate
 - Unconjugated
 - Vial size
 - 0.08 ml, 0.4 ml
 - Concentration
 - In 1X PBS, pH 7.4, with 0.09% sodium azide
 - Storage
 - Aliquot the XRCC6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
 
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					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Western blot analysis of XRCC6 antibody and A2058 lysate.
 
- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Western blot analysis of XRCC6 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the XRCC6 gene.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Fluorescent confocal image of HeLa cells stained with XRCC6 antibody. Alexa Fluor 488 secondary (green) was used. XRCC6 immunoreactivity is localized to the nucleus strongly and cytoplasm weakly.
 
- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Confocal immunofluorescent analysis of XRCC6 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
 
							
					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - XRCC6 antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - XRCC6 antibody flow cytometric analysis of A2058 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.