NBP2-34247-0.1mg

antibody from Novus Biologicals

Targeting: XRCC6 D22S671, D22S731, G22P1, KU70, ML8

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-34247-0.1mg

Provider

Novus Biologicals

Product name

Mouse Monoclonal Ku70/XRCC6 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

0.2 mg/ml

Storage

Store at 4C.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Ku Monoclonal Antibody (KU729).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometric Analysis of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ku70/XRCC6 Antibody (KU729).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Azide and BSA Free format of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Azide and BSA Free format of this antibody.