M01732-1
antibody from Boster Biological Technology
		Targeting: XRCC6
		
		D22S671, D22S731, G22P1, KU70, ML8	
	
	
	
	
Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [1]
 
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Validation data
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- Product number
 - M01732-1 - Provider product page

 - Provider
 - Boster Biological Technology
 - Product name
 - Anti-Ku70 XRCC6 Rabbit Monoclonal Antibody
 - Antibody type
 - Monoclonal
 - Description
 - Monoclonal antibody for Ku70/XRCC6 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human Ku70/XRCC6 information: Molecular Weight: 69843 MW; Subcellular Localization: Nucleus . Chromosome .
 - Reactivity
 - Human
 - Host
 - Rabbit
 - Antibody clone number
 - AAIG-24
 - Vial size
 - 100ug/vial
 - Storage
 - At -20°C for one year. Avoid repeated freezing and thawing.
 
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					Supportive validation
					
									
				
		- Submitted by
 - Boster Biological Technology (provider)
 - Main image
 
- Experimental details
 - Western blot analysis of Ku70 expression in HeLa cell lysate (M01732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC6 monoclonal antibody (Catalog # M01732-1) overnight at 4
 - Additional image
 