PA5-27538

antibody from Invitrogen Antibodies

Targeting: XRCC6 D22S671, D22S731, G22P1, KU70, ML8

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27538

Provider

Invitrogen Antibodies

Product name

Ku70 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (82%), Rat (83%), Pig (87%), Chimpanzee (100%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.25 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Rad51 recruitment and exclusion of non-homologous end joining during homologous recombination at a Tus/Ter mammalian replication fork barrier.

Willis NA, Panday A, Duffey EE, Scully R
PLoS genetics 2018 Jul;14(7):e1007486

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The interaction of adenovirus E1A with the mammalian protein Ku70/XRCC6.

Frost JR, Olanubi O, Cheng SK, Soriano A, Crisostomo L, Lopez A, Pelka P
Virology 2017 Jan;500:11-21

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Ku70 in HeLa cells fixed with 4% paraformaldehyde at RT for 15 min. Green: Ku70 Polyclonal Antibody (Product # PA5-27538) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Ku70 in HeLa cells fixed with 4% paraformaldehyde at RT for 15 min. Green: Ku70 Polyclonal Antibody (Product # PA5-27538) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Ku70 Polyclonal Antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 µg of Ku70 Polyclonal Antibody (Product # PA5-27538) 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 Polyclonal Antibody (Product # PA5-27538) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70 (Product # PA5-27538) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Ku70 was performed in paraffin-embedded human cervical cancer tissue using Ku70 Polyclonal Antibody (Product # PA5-27538) at a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Ku70 Polyclonal Antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 µg of Ku70 Polyclonal Antibody (Product # PA5-27538) 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 Polyclonal Antibody (Product # PA5-27538) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 6 Transient KU70 expression does not affect Tus/ Ter -induced HR in Ku70 -/- cells. A , Frequencies of Tus/ Ter -induced and I-SceI-induced repair in four independently derived Ku70 -/- 6x Ter -HR reporter clones (clones 27, 34, 41, and 47) with and without transient expression of exogenous hKU70 . Cells transiently co-transfected with empty pcDNA3beta or pcDNA3beta- hKU70 expression vector and either empty, 3xMyc-NLS Tus or 3xMyc-NLS I-SceI expression vectors. Each column represents the mean of duplicate samples from eight independent experiments (n = 8), values are corrected for transfection efficiency. Error bars: s.e.m. Tus-induced total HR, t test: #27 +EV vs. + hKU70 , p = 0.8355; #34 +EV vs. + hKU70 , p = 0.3799; #41 +EV vs. + hKU70 , p = 0.2710; #47 +EV vs. + hKU70 , p = 0.4703; Tus-induced STGC, t test: #27 +EV vs. + hKU70 , p = 0.7892; #34 +EV vs. + hKU70 , p = 0.4223; #41 +EV vs. + hKU70 , p = 0.2345; #47 +EV vs. + hKU70 , p = 0.4426; Tus-induced LTGC, t test: #27 +EV vs. + hKU70 , p = 0.4375; #34 +EV vs. + hKU70 , p = 0.2413; #41 +EV vs. + hKU70 , p = 0.8608; #47 +EV vs. + hKU70 , p = 0.9872; Tus-induced LTGC/(Total HR), t test: #27 +EV vs. + hKU70 , p = 0.2701; #34 +EV vs. + hKU70 , p = 0.4964; #41 +EV vs. + hKU70 , p = 0.2507; #47 +EV vs. + hKU70 , p = 0.8222. I-SceI-induced total HR, t test: #27 +EV vs. + hKU70 , p