- PA5-27538 - Invitrogen Antibodies XRCC6 antibody

Willis NA, Panday A, Duffey EE, Scully R
PLoS genetics 2018 Jul;14(7):e1007486

Frost JR, Olanubi O, Cheng SK, Soriano A, Crisostomo L, Lopez A, Pelka P
Virology 2017 Jan;500:11-21

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Ku70 using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Ku70 polyclonal antibody (Product # PA5-27538) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Ku70 using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Ku70 polyclonal antibody (Product # PA5-27538) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Ku70 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Ku70 Polyclonal Antibody (Product # PA5-27538) at a dilution of 1:2000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Ku70 was achieved by transfecting HeLa cells with Ku70 specific siRNAs (Silencer® select Product # s5457, s5455). Western blot analysis (Fig. a) was performed using nuclear enriched cell extracts from the Ku70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Ku70 Polyclonal Antibody (Product # PA5-27538, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Ku70.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). The blot was probed with Anti-Ku70 Polyclonal Antibody (Product # PA5-27538, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 70kDa band corresponding to Ku70 was observed across all the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of Ku70 in HeLa cells fixed with 4% paraformaldehyde at RT for 15 min. Green: Ku70 Polyclonal Antibody (Product # PA5-27538) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of Ku70 was performed in paraffin-embedded human cervical cancer tissue using Ku70 Polyclonal Antibody (Product # PA5-27538) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70 (Product # PA5-27538) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Ku70 Polyclonal Antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 µg of Ku70 Polyclonal Antibody (Product # PA5-27538) 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 Polyclonal Antibody (Product # PA5-27538) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 6 Transient KU70 expression does not affect Tus/ Ter -induced HR in Ku70 -/- cells. A , Frequencies of Tus/ Ter -induced and I-SceI-induced repair in four independently derived Ku70 -/- 6x Ter -HR reporter clones (clones 27, 34, 41, and 47) with and without transient expression of exogenous hKU70 . Cells transiently co-transfected with empty pcDNA3beta or pcDNA3beta- hKU70 expression vector and either empty, 3xMyc-NLS Tus or 3xMyc-NLS I-SceI expression vectors. Each column represents the mean of duplicate samples from eight independent experiments (n = 8), values are corrected for transfection efficiency. Error bars: s.e.m. Tus-induced total HR, t test: #27 +EV vs. + hKU70 , p = 0.8355; #34 +EV vs. + hKU70 , p = 0.3799; #41 +EV vs. + hKU70 , p = 0.2710; #47 +EV vs. + hKU70 , p = 0.4703; Tus-induced STGC, t test: #27 +EV vs. + hKU70 , p = 0.7892; #34 +EV vs. + hKU70 , p = 0.4223; #41 +EV vs. + hKU70 , p = 0.2345; #47 +EV vs. + hKU70 , p = 0.4426; Tus-induced LTGC, t test: #27 +EV vs. + hKU70 , p = 0.4375; #34 +EV vs. + hKU70 , p = 0.2413; #41 +EV vs. + hKU70 , p = 0.8608; #47 +EV vs. + hKU70 , p = 0.9872; Tus-induced LTGC/(Total HR), t test: #27 +EV vs. + hKU70 , p = 0.2701; #34 +EV vs. + hKU70 , p = 0.4964; #41 +EV vs. + hKU70 , p = 0.2507; #47 +EV vs. + hKU70 , p = 0.8222. I-SceI-induced total HR, t test: #27 +EV vs. + hKU70 , p

PA5-27538