Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX48821 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48821, RRID:AB_11176640
- Product name
- Sumo antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot of hSUMO fusion protein. Anti-SUMO antibody, generated by immunization with recombinant human SUMO, was tested by western blot against a SUMO-GFP fusion protein after cleavage by proteases. Dilution of the antibody between 1:1,000 and 1:5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead). In this blot the antibody was used at a 1:2000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of hSUMO fusion protein. Anti-SUMO antibody, generated by immunization with recombinant human SUMO, was tested by western blot against a SUMO-GFP fusion protein after cleavage by proteases. Dilution of the antibody between 1:1,000 and 1:5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead). In this blot the antibody was used at a 1:2000 dilution incubated overnight at 4¢X C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG