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LearnAF3289
antibody from Novus Biologicals
Targeting: SUMO1
GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF3289 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal SUMO1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human and mouse proteins modified with SUMO1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) SUMO2 and rhSUMO3 and less than 1% cross-reactivity with rhSUMO4 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SUMO1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, DU145 human prostate carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SUMO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3289) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
