Explore
Validate
LearnA-714
antibody from Novus Biologicals
Targeting: SUMO1
GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A-714 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal SUMO Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. This antibody detects endogenous, human SUMOylated proteins in Western blots, and has similar sensitivity when tested against purified, recombinant SUMO1, SUMO2, SUMO3 and SUMO4.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store the unopened product at -20 degreesC. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 degreesC is not recommended. Do not use past expiration date.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SUMO by Western Blot. Western blot shows lysates of pan-SUMO. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO Pan Specific Monoclonal Antibody (Catalog # A-714) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
