PA5-34765

antibody from Invitrogen Antibodies

Targeting: SUMO1 GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1

Antibody data

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Validation data

Product number: PA5-34765 - Provider product page
Provider: Invitrogen Antibodies
Product name: SUMO1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: The recommended shelf life for this product is 1 year from date of receipt.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SUMO1 protein structure - PA5-34765 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SUMO1 in paraformaldehyde-fixed U2OS cells using a SUMO1 polyclonal antibody (Product # PA5-34765) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SUMO1 (Small ubiquitin-related modifier 1) was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SUMO1 Polyclonal Antibody (Product # PA5-34765) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing punctate nuclear and cytoskeletal staining localization for SUMO1 in Hep G2 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification with CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7C1115LZR).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SUMO1 was achieved by transfecting Hep G2 cells with SUMO1 specific siRNA (Silencer® select Product # s14608, s14609). Immunofluorescence analysis was performed on untransfected Hep G2 cells, transfected with non-specific scrambled siRNA and transfected with SUMO1, SUMO2 and SUMO3 siRNAs, separately. Cells were fixed, permeabilized, and labelled with SUMO1 Polyclonal Antibody (Product # PA5-34765, 1:100) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (Red) staining. Reduction of specific punctate nuclear signal was observed upon siRNA mediated knockdown of SUMO1 only and not SUMO2 or SUMO3, confirming specificity of the antibody to SUMO1 (Green). The images were captured at 60X magnification with EVOS™ M7000 Imaging System (Product # AMF7000).