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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324522 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal SUMO1 Antibody (N-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal SUMO1 Antibody (N-term)
- Reactivity
- Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SUMO1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.46 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SUMO1 Antibody (N-term) (Cat. #TA324522) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SUMO1 antibody detected the SUMO1 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from mouse Neuro-2a cell line, using SUMO1 Antibody (N-term) (Cat. #TA324522). TA324522 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of A549 cell stained with SUMO1 Antibody (N-term)(Cat#TA324522).A549 cells were incubated with SUMO1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).SUMO1 immunoreactivity is localized to Nuclear Membrane significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SUMO1 Antibody (N-term) (Cat. #TA324522)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SUMO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
