F42008
antibody from NSJ Bioreagents
Targeting: SUMO1
GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F42008 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SUMO Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 55-86 from human SUMO1 was used as the immunogen for this SUMO antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the SUMO antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Top: lysate tested with ERM Ab. Center: IP with FLAG Ab followed by WB with NSJ# F42008 SUMO antibody. Bottom: IP with FLAG Ab followed by WB with NSJ# F42027 SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms detected by SUMO Abs.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- WB: SUMO polyclonal antibody used in western blot to detect GST-SUMO1 fusion protein. Predicted molecular weight: 12-15 kDa + 25 kDa GST tag.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human breast carcinoma tissue stained with the SUMO antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SUMO antibody flow cytometric analysis of HeLa cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.