ABIN500837

antibody from antibodies-online

Targeting: SUMO1 GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN500837

Provider

antibodies-online

Product name

anti-SMT3 Suppressor of Mif Two 3 Homolog 1 (S. Cerevisiae) (SUMO1) (N-Term) antibody

Antibody type

Polyclonal

Antigen

Sumo antibody was raised against a 14 amino acid peptide from near the amino terminus of human sumo.

Description

Peptide affinity chromatography

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

N-Term

Isotype

IgG

Vial size

0.1 mg

Storage

Store at 2 - 8°C for up to one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

SUMO protein modification.

Dohmen RJ
Biochimica et biophysica acta 2004 Nov 29;1695(1-3):113-31

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SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation.

Desterro JM, Rodriguez MS, Hay RT
Molecular cell 1998 Aug;2(2):233-9

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A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.

Matunis MJ, Coutavas E, Blobel G
The Journal of cell biology 1996 Dec;135(6 Pt 1):1457-70

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of sumo in HL-60 cell lysate with AP30842PU-N at (A) 0.5, (B) 1, and (C) 2 ?g/ml.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunocytochemistry of Sumo in HL60 cells with AP30842PU-N at 5 ?g/ml.