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LearnR1197
antibody from Acris Antibodies GmbH
Targeting: SUMO1
GMP1, OFC10, PIC1, SMT3C, SMT3H3, SUMO-1, UBL1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- R1197 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1197, RRID:AB_1007582
- Product name
- anti SUMO1
- Antibody type
- Polyclonal
- Antigen
- This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.5 mg
- Concentration
- 5.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Immunoblot of hSUMO fusion protein.Anti-SUMO antibody, generated by immunizationwith recombinant human SUMO, was tested byimmunoblot against a SUMO-GFP fusion proteinafter cleavage by proteases. Dilution of theantibody between 1:1,000 and 1:5,000 showedstrong reactivity specifically with the SUMO portion of the fusion protein (arrowhead). In this blot theantibody was used at a 1:2000 dilution incubatedovernight at 4° C in 5% non-fat dry milk in TTBS.Detection occurred using a 1:2000 dilution of HRPlabeledDonkey anti-Rabbit IgG for 1 hour at room temperature. Achemiluminescence system was used for signaldetection (Roche). Other detection systems willyield similar results. Data contributed by M.Malakhov, www.lifesensors.com, personalcommunication.
