sc-205

antibody from Santa Cruz Biotechnology

Targeting: PLCB1 KIAA0581, PLC-I, PLC154

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-205

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-205, RRID:AB_632196

Product name

Anti-PLCB1

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Suppression of PLCbeta2 by endotoxin plays a role in the adenosine A(2A) receptor-mediated switch of macrophages from an inflammatory to an angiogenic phenotype.

Grinberg S, Hasko G, Wu D, Leibovich SJ
The American journal of pathology 2009 Dec;175(6):2439-53

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Increased Expression and Activity of Phospholipase C in Renal Arterioles of Young Spontaneously Hypertensive Rats^*

Zhangping Peng, An Dang, William J. Arendshorst
American Journal of Hypertension 2007 Jan;20(1):38-43

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 25

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.